| dc.description.abstract | Main target of this PhD project was to define the metabolome of the main by-products, and in certain
cases of the edible parts, of selected agroalimentary productions, that are the flowers of Opuntia ficus
indica Mill. (nopal cactus), the leaves of Ficus carica L. (common fig), the leaves, the husks, the
shells and the kernels of Prunus dulcis Mill. (sweet almond) and Pistacia vera L. (pistachio).
Attention was mainly focused on valorizing the waste material generated from manufacturing
processes as potential sources of bioactives, pointing out their perspective employment in nutraceutics
and cosmetics.
Particularly, the selected plant parts were extracted by employing different solvents and extraction
methods. The obtained extracts were submitted to LC-HRMS/MS experiments, in order to achieve a
preliminary overview on their chemical composition. Polar extracts were fractionated by different
chromatographic approaches, and the isolated compounds were characterized by 1D and 2D-NMR
experiments, further supported by HRMS experiments. In addition, total phenolic content was
determined by Folin-Ciocalteu assay, while radical scavenging activity was evaluated by DPPH and
ABTS assays. Moreover, the main constituents of certain extracts were quantified by LC-MS/MS
experiments by a Multiple Reaction Monitoring approach.
The obtained results highlighted sweet almonds and pistachios as rich sources of unsaturated fatty
acids and antioxidant phenolics. On the other hand, the main by-products of the selected species
exhibited a variegated metabolome, with several constituents belonging to different chemical classes,
mainly phenolics, reported for their antioxidant and antinflammatory properties, suggesting their
potential employment for the manufacturing of nutraceutical and cosmetic formulations. [edited by Author] | it_IT |
